Phenotypeca utilises a stable whole 2-micron expression vector that is exchangeable allowing the matching of the optimised expression vector (best promoter, pre- and pro-leader, terminator and expression optimised codons) with the optimised strain (combination of genetic variants that provide beneficial outcomes). Unlike vectors in use by academia and many industries, the whole 2-micron vector is stably maintained at high copy number over many generations with little cell to cell variation. This provides advantages over integrated expression constructs in terms of copy number, stability (when multiple copies are integrated) and interchangeability. Our team matches your protein sequence (expression optimised using proprietary algorithms) with appropriate regulatory and signal and leader sequences to be assayed in a set of key strains that have been optimised and engineered for various aspects of heterologous protein production. Proper leader processing can be enhanced with appropriate target sequences of the proteases involved as well as regulating the protease.